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By triggering an imbalance in redox homeostasis, mitochondrial dysfunction not only impairs electron transport chain activity but also disrupts the dynamic balance between organelles, leading to ultrastructural abnormalities (e.g., mitochondrial cristae rupture, endoplasmic reticulum vesiculation) and oxidative damage to key biomolecules in oocytes, which, in turn, significantly reduces the developmental potential of oocytes and the cytoplasm of oocytes

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Withdraw the needle smoothly, apply light pressure with a clean swab if any bleeding occurs, and dispose of the needle properly