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For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig

Patients are left with recurring, crippling symptoms, which could be avoided if treatment continued

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Within this cohort, a significant proportion arrive at treatment with secondary systemic compromise that extends beyond the primary viral pathology