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doi:10.1016/j.juro.2009.11.034

BAC water added 1.0 mL Concentration 5 mg/mL (5,000 mcg/mL) 300 mcg dose 0.06 mL / 6 units 500 mcg dose 0.10 mL / 10 units BAC water added 2.0 mL Concentration 2.5 mg/mL (2,500 mcg/mL) 300 mcg dose 0.12 mL / 12 units 500 mcg dose 0.20 mL / 20 units BAC water added 3.0 mL (recommended for accuracy) Concentration ~1.667 mg/mL (1,667 mcg/mL) 300 mcg dose 0.18 mL / 18 units 500 mcg dose 0.30 mL / 30 units 3.0 mL is the most common research-context dilution because it pushes daily draws above 10 syringe units, which makes measurement on a 0.3 mL U-100 syringe easier

Yes the higher total quantity allows researchers to set up concentration gradients across a wider range than a single standard-dose vial would permit, making it a practical choice for EC50/IC50-type studies or dose escalation protocols

ns, not significant