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Particularly for heart failure, several clinical trials and meta-analyses have already confirmed that SGLT2is reduce HHF by approximately 30% in both HFrEF and HFpEF, with or without diabetes [46]

For immunofluorescence, the primary antibodies used were monoclonal [8G9] to GLP-1, monoclonal SST antibody (#MAB2358), and monoclonal Human/Mouse Ghrelin Antibody (R&D systems #MAB8200), and the secondary antibodies were Alexa Fluor 488 AffiniPure Donkey Anti-Rat IgG and Cyanine Cy3 AffiniPure Donkey Anti-Mouse IgG from Jackson ImmunoResearch (Europe Ltd.) and were used at a final dilution of 1/300