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Finally, BALF scRNA-seq data from air control and smoke-exposed samples of the acute, sub-chronic and chronic smoke models were integrated into a single object for integrative analysis

Although the directionality of these alterations may depend on the cell type, disease status or time or dose of exposure, this collective body of evidence demonstrates that the molecular regulation of mitochondrial biogenesis is disturbed in COPD patients and is impacted by smoking in epithelial cells of both the bronchial and alveolar compartment

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