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ImageJ 1.51 s (National Institutes of Health, Bethesda, MD, USA) was used for the evaluation of single mitochondrial morphological parameters (area, perimeter, circularity, Ferrets diameter, aspect ratio, and roundness)

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We further computed the correlations between the differential genera and plasma factors in smoking-related enterotypes of each case group to preliminarily explain whether the changes in bacterial abundance are related to the role of inflammation and intestinal barrier function