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The samples were centrifuged at 4C, 2300x g for 5 min and the serum transferred into polypropylene microcentrifuge tubes

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The animal literature is unusually consistent, though: nearly every published in vivo study used 5 mg/kg per injection, given three times on alternate days (days 1, 3, and 5, or days 14, 16, and 18 in the pulmonary fibrosis model), with effects tracked over the following three to four weeks