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The RNA-seq data was processed using the TrimGalore toolkit [1] which employs Cutadapt [2] to trim low-quality bases and Illumina sequencing adapters from the 3 end of the reads

Conclusion Despite there being an established risk between prenatal maternal smoking and adverse outcomes, as of 2016 one in fourteen women (7.2%) in the United States reported to have smoked cigarettes during their pregnancy with the global prevalence of smoking during pregnancy estimated to be 1.7% [ In conclusion, the results of this review suggest the importance of ceasing smoking and any form of nicotine exposure during pregnancy to mitigate the risk of long-lasting complications on offspring

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