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Homogenates were centrifuged at 48,000 g (50 min, 4 C) and washed twice in the same conditions to isolate the membrane fraction

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This delayed onset is a direct consequence of buprenorphines long half-life of 24 to 42 hours, which means the drug clears from the body far more slowly than short-acting opioids like oxycodone, which has a half-life of only three to four hours