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Briefly, hASM cells plated in eight-well chambered coverslips (Ibidi, Grafelfing, DEU) were incubated in Hanks balanced salt solution (HBSS) containing 1 M Rhod 2-AM (Thermo Fisher Scientific, Waltham, MA) for 45 min at room temperature, medium aspirated, and washed three times in HBSS and incubated further for 15 min to allow for deesterification of the Rhod 2

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Smoking Smarties is a party trick where someone crushes a pack of Smarties candy into powder, inhales it gently, and exhales it out through their nose or mouth to make it look like theyre smoking a cigarette