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Cells were then washed twice with PBS containing 10% serum and fixed with 70% ethanol overnight at 20 C

The (KLAKLAK)2 peptide encapsulated into mesoporous nanoparticles induced mitochondrial swelling and apoptosis 51

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Building a MOTS-C Protocol Within a Longevity Program A MOTS-c protocol is most powerful not as a standalone intervention but as one layer of a structured longevity program that addresses mitochondrial health, metabolic flexibility, and body composition simultaneously