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Tobacco smoke contains over 4000 potential toxicants, including nicotine, which is an addictive alkaloid and a natural insecticide of tobacco plants, Nicotiana spp
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Other predisposing factors include: poor oral hygiene, smoking, oxidizing mouthwashes, oral antibiotics, excessive coffee or tea drinking, and conditions that suppress the immune system, such as HIV or cancer
Each eluent subjected to Gas chromatography-thermal energy analysis (GC-TEA) (Health Canada T-111) for analysis of TSNAs including Nnitrosonornicotine (NNN), 4-[methylnitrosamino]-1-[3- pyridyl]-1-butanone (NNK), N-nitrosoanatabine (NAT) and Nnitrosoanabasine (NAB) [25]
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