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Following immunoprecipitation, the bead-bound SNAP/FLAG-hGLP-1R was incubated with a click chemistry mix (20 M rhodamine-azide, 1 mM TCEP, 100 M TBTA, 1 mM CuSO 4 ) for 1 hour at room temperature gently agitating to fluorescently label the PhotoClick cholesterol

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For the scavenging experiment, cells were pre-incubated with media having TB m A-Glu supplemented with ROS scavengers (Trolox: 100 M, mannitol: 50 mM, Tiron: 10 mM, NaN 3 :5 mM) for 12 h before light irradiation (12 Jcm 2 )