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These items are also more appropriate for the Indian context than some items from the GN-STBQ 11 and BQDS 15 due to a lack of policies restricting the use of smokeless tobacco in public places 27 and easy accessibility of tobacco shops 28 in India

Then cDNA synthesis was performed using Revertaid cDNA synthesis kit with random hexamer primers (Thermo Scientific), 1ng was used for RT-qPCR analysis of MT-CYB , MT-CO2 and MT-RNR1

da Costa e Silva noted