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ALI cultures were fixed with a solution of 50%/50% methanol/acetone for 2 min at 20 C followed by three washes with PBS

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The court does not find this argument to be persuasive. The coupon ban also applies to other types of tobacco products, and Greisas ruling rejects a request by the plaintiffs which include the National Association for Tobacco Outlets and tobacco giants RJ Reynolds and Philip Morris for a permanent injunction preventing the city from enforcing the law

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