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With long-term exercise, there seems to be some reduction in energy expenditure from other elements of metabolism that overall reduces the amount of extra Calories being burned during exercise, and results in only modest changes to total daily energy expenditure

While acute supplementation of 4.5 grams was previously shown to provide significant enhancement of anaerobic work capacity, the present study suggests that chronic supplementation of GPLC at 3.0 or 4.5 grams daily does not improve anaerobic performance of repeated high speed high intensity bouts and may actually produce detrimental effects with high velocity, high intensity exercise

Endogenous phenotype indicators include changes in RNAs (e.g., mRNAs and miRNAs), proteins or metabolites

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