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This was an adaptation of the protocol described by Neilson and collaborators ( In our case, having used a medium with poor ingredients, with respect to the protocol described in literature ( in vivo study was performed in two phases, first using a wild-type mouse ( Mus musculus ) model, C57BL/6 (FMO3 +/+ ) female mice, where TMAO levels in the blood were measured 3 h after the administration of the inhibitor and 2 hours after the administration of choline orally

Cas9, guided by sgRNAs targeting Acsl4 (5-TGAAATGCAGCCAAATGGAA-3) or Fsp1 (5-TGCACGTGGTGATCGTGGGC-3) [44], mediated cleavage of these genes

There is no universal safest dosage

Colonies were then trnsferred to SD/-Trp/-Leu/-His/-Ade selective medium and incubated for an additional 72 h to enable imaging and documentation of interaction events