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After three basal measurements, cells were permeabilized by injection of saponin (1 g/mL, Sigma S4521), together with 1 mM ADP (Sigma 01905) and the following respiratory complex substrates: CI, pyruvate/malate (5 mM/2.5 mM)

The continuous exposure of RBCs to ROS can cause cell damage, including lipid and protein oxidation, causing damages in enzymes and ion transport proteins [19, 24]