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MTT assay The number of viable cells was measured with the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT, SigmaAldrich, USA) assay (Hansen et al

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In addition, HSF1 activity is not directly responsible for HSC70 but rather for HSPA1 activity, while the degradation of GPX4 is HSC70 dependent