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To maximize the capture of individual DUBs, we pooled probes from three different classes that included a mono-ubiquitin recognition element specific for DUBs, a biotin-tag for DUBs pulldown, and either a propargylamide (PA), a vinylmethylester (VME), or a vinylsulfone (VS) electrophilic warhead to covalently interact with the nucleophilic cysteine residue in the active sites of DUBs 15,16,17 (Fig