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Using C2C12 mouse myoblasts, the study confirmed that the MGF E-domain peptide increases myoblast proliferation while inhibiting terminal differentiation and critically, that this effect was maintained in the presence of a neutralising anti-IGF-1R antibody directly establishing that MGF E-peptide signals via an IGF-1R-independent mechanism

Relevance to tissue-repair research more broadly IGF-1 signaling intersects with the wider field of musculoskeletal repair research, where a range of peptides are studied for their proposed effects on connective tissue, tendon, and muscle