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These studies reveal that islet-derived proglucagon products are the predominant ligand for beta-cell glucagon-like peptide 1 receptor (GLP-1R) activation and are necessary for the glucoregulatory improvements of DPP-IV inhibitors (DPP-IVi) and vertical sleeve gastrectomy (VSG) ( In further characterizing this paracrine effect, the field has employed several different models of selective inhibition of proglucagon expression to more directly investigate the relative contributions of gut- versus islet-derived proglucagon products to glucose homeostasis ( Figure 2 )

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Plant Physiol 134:17841792 Barth C, Tullio MD, Conklin PL (2006) The role of ascorbic acid in the control of flowering time and the onset of senescence

Figure 6 3.8 RT-qPCR validation of transcriptomic results RT-qPCR analysis validated the reliability of the RNA-seq data, demonstrating consistent trends in gene expression between the two methods