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Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate

While benign, the urinary discolouration may interfere with colorimetric laboratory analyses, and may impede intermittent haemodialysis due to the pigment distribution across the dialysate membrane triggering colorimetric blood leak sensors

A consultation before treatment helps identify whether glutathione injections are appropriate based on personal health history and skincare goals

Mild Systemic Effects Some users experience temporary headaches or mild digestive upset