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In vitro studies using cell cultures like Human Umbilical Vein Endothelial Cells (HUVECs) are employed to examine effects on cell migration, proliferation, and tube formationkey events in the formation of new blood vessels

This is often due to limited dietary intake, malabsorption, medical conditions, or the use of B12-depleting medications ( Vitamin B12 deficiency is determined as follows ( Because the ability to absorb B12 from food declines with age, deficiency is more common in older adults

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